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C18 displays the typical options on the alkyl team - non-polar and ionically inert in most commonly encountered circumstances. Retention from the C18 stationary section comes from weak van der Waals intermolecular drive that pulls hydrophobic compounds. C18 together with other alkyl phases like C8 and C4 represent probably the most basic style of r
In several cases, baseline separation of your peaks may be attained only with gradient elution and small column loadings. As a result, two drawbacks to elution manner chromatography, Particularly on the preparative scale, are operational complexity, as a consequence of gradient solvent pumping, and small throughput, due to low column loadings. Disp
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